in camel keds collected from camel herds in various geographical locations in Laisamis, northern Kenya.Īnaplasmosis, caused by infection with bacteria of the genus Anaplasma, is an important veterinary and zoonotic disease.
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Table E in S1 Text: Presence of Anaplasma sp. Anaplasma camelii’ in camels and camel keds in various seasons of the year wet, late wet, and dry season. Table D in S1 Text: The infection prevalence of ‘ Ca. Control mice ( n = 8) and rabbits ( n = 2) were not exposed to biting flies. PCR-HRM analysis targeting genus-specific 16S rRNA gene detected Anaplasma infection rates of 17.9% in test mice ( n = 22/123) and in 25% of rabbits ( n = 1/4) post-ked bites. camelina, on mice ( n = 123) and rabbits ( n = 6) for Anaplasma transmission study to determine vector competence of keds. Table C in S1 Text: The feeding schedule of camel keds, H. We recorded Anaplasma infection rate of 12.9% ( n = 4/31) in mice after 60 days of follow-up screening, but all 27 mice that were sacrificed 140 days post-ked exposure were not infected with the Anaplasma sp. Control mice were not exposed to fly bites. PCR-HRM analysis revealed Anaplasma infection rate of 6.9% in test mice ( n = 4/58) two weeks post-ked bite exposure. camelina, on immunosuppressed mice ( n = 60) for Anaplasma transmission to determine the effect of immunosuppression on mice infection. Table B in S1 Text: The feeding schedule of camel keds, H. The control mice group ( n = 2) was not exposed to the biting flies.
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The data shows that 47.4% of mice in the test group ( n = 9/19) have acquired Anaplasma infection following ked blood-feeding bites. Anaplasma camelii’ in experimental mice group was determined post-ked bites by PCR-HRM using genus-specific primers for 16S rRNA gene target. S1 Text: Table A in S1 Text: The feeding schedule of camel keds, Hippobosca camelina, on healthy Swiss white mice for pathogen transmission experiment. Masiga, Conceptualization, Formal analysis, Funding acquisition, Methodology, Resources, Supervision, Validation, Writing – review & editing Mark Carrington, Conceptualization, Formal analysis, Funding acquisition, Methodology, Resources, Supervision, Validation, Writing – review & editing,Īnd Daniel K. Muema, Formal analysis, Writing – review & editing, Copeland, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Writing – review & editing, Jandouwe Villinger, Formal analysis, Resources, Supervision, Writing – review & editing, Getahun, Formal analysis, Resources, Supervision, Writing – review & editing, Kidambasi, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing, Bargul, Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing, Added layers merge functionality.ĭownload: Master PDF Editor 5.0.36 | 29.4 MB (Shareware)ĭownload: Master PDF Editor for Linux | 66.8 MBĭownload: Master PDF Editor for macOS | 26.Joel L. Changing font attributes (size, family, color etc).įixed issues with Import and Export of PDF pages.Signing PDF documents with digital signature, signatures creation and validation.
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